Terminal heat stress-responsive genes analysis in heat susceptible HDR77 genotype of wheat (Triticum aestivum L.) by using semi-quantative RTPCR
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چکیده
منابع مشابه
Characterization and Expression of High Temperature Stress Responsive Genes in Bread Wheat (Triticum aestivum L.)
To elucidate the effects of high temperatures, wheat plants (Triticum aestivum cv. CPAN 1676) were given heat shocks at 37°C and 42°C for two hours, and responsive genes were identified through PCR-Select Subtraction technology. Four subtractive cDNA libraries, including three forward and one reverse subtraction, were constructed from three different developmental stages. A total of 5500 ESTs w...
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Heat-responsive miRNAs regulate the expression of the transcription factors (HSFs) and majority of the heat stress-associated genes (HSPs). Here we report identification of few heats responsive miRNAs in wheat (Triticum aestivum L.)through de novo sequencing on Illumina Hiseq 2000. Validation of identified miRNAs in endospermic tissues of thermotolerant (HD2985) and thermosusceptible (NIAW-34-3...
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Heat stress is one of the major problems in wheat growth and yield. It causes denaturation and aggregation of key enzymes involved in different pathways and affects the quality of the grains. The defence mechanism associated with thermotolerance in wheat is not known and very few stress associated proteins has been characterised. We performed an extensive identification of heat-responsive prote...
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Eight spring wheat cultivars were evaluated under three heat stress conditions (early, late and very late) in order to identify suitable cultivars to develop heattolerant genotypes resistant to future global warming. Results from the study indicate that stress did not negatively affect flag leaf area in ‘Prodip’ and ‘Sufi’, flag leaf dry matter partitioning in ‘Prodip’, ‘BARI Gom-26’ and ‘S...
متن کاملCharacterization of differentially expressed stress-associated proteins in starch granule development under heat stress in wheat (Triticum aestivum L.).
Abiotic stress causes abrupt increase in the expression of stress-associated proteins, which provide tolerance by modulating the defense mechanism of plants. Small heat shock proteins (sHSPs) and anti-oxidant enzymes are important for environmental stress tolerance of the plants. In this study, two full-length cDNAs encoding small heat shock protein (sHSP) and superoxide dismutase (SOD), design...
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ژورنال
عنوان ژورنال: Electronic Journal of Plant Breeding
سال: 2017
ISSN: 0975-928X
DOI: 10.5958/0975-928x.2017.00164.8